نتایج جستجو برای: gus gene

تعداد نتایج: 1142251  

Journal: :علوم گیاهان زراعی ایران 0
مسعود توحیدفر لیلا رمضان‏پور ریحانه گلابچیان

production of transgenic cotton (gossypium hirsutum l.) was studied utilizing agrobacterium-mediated strain lba4404 and pbi121 plasmid. this plasmid contains gus as a reporter gene driven by the camv35s promoter and nptii gene driven by nos as selection marker. in this study, the inheritance of gus gene was evaluated using selective media, pcr and gus staining in the second genertaion (t1) of t...

In this study, an efficient Agrobacterium-mediated transformation method was developed forpomegranate (Punica granatum L.), a difficult-to-transform plant. In vitro shoot segments wereinoculated with Agrobacterium tumefaciens strain LBA4404 harboring the binary vectorpBI121 carrying the neomycin phosphotransferase (nptII) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...

Journal: :Plant physiology 2004
Bunichi Ezaki Masakatsu Suzuki Hirotoshi Motoda Masako Kawamura Susumu Nakashima Hideaki Matsumoto

The gene expression of two Al-induced Arabidopsis glutathione S-transferase genes, AtGST1 and AtGST11, was analyzed to investigate the mechanism underlying the response to Al stress. An approximately 1-kb DNA fragment of the 5'-upstream region of each gene was fused to a beta-glucuronidase (GUS) reporter gene (pAtGST1::GUS and pAtGST11::GUS) and introduced into Arabidopsis ecotype Landsberg ere...

Journal: :journal of agricultural science and technology 2014
s. c. liu g. c. zhang l. f. yang m. mii j. y. gai

a stable agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [glycine max (l.) merr.]. the cotyledonary node explants of the soybean cultivar ‘ny-1001’ were inoculated with the agrobacterium tumefaciens strain eha105, harboring the vector pcambia3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...

Journal: :international journal of horticultural science and technology 2014
babak valizadeh kaji ahmad ershadi masoud tohidfar

in this study, an efficient agrobacterium-mediated transformation method was developed forpomegranate (punica granatum l.), a difficult-to-transform plant. in vitro shoot segments wereinoculated with agrobacterium tumefaciens strain lba4404 harboring the binary vectorpbi121 carrying the neomycin phosphotransferase (nptii) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

Journal: :Journal of bioscience and bioengineering 2011
Hossein Kamaladini Siti Nor Akmar Abdullah Maheran Abdul Aziz

Reporter gene activity under the regulation of the oil palm metallothionein-like gene, MT3-A promoter was assessed in prokaryotes. Vector constructs containing MT3-A promoter with (W1MT3-A) and without (W2MT3-A) five prime untranslated region (5'-UTR) fused to ß-glucuronidase (GUS) gene in pCAMBIA 1304 vector were produced. 5'-rapid amplification of cDNA ends (RACE) using mRNA isolated from Esc...

Journal: :The Plant cell 2003
Ludmila Mlynárová Andrea Hricová Annelies Loonen Jan-Peter Nap

We present isogenic transgenic tobacco lines that carry at a given chromosomal position a beta-glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations ...

2011
Akio OHYAMA Koji TANASE Keita SUWABE Miyuki KUNIHISA Tsukasa NUNOME Hiroyuki FUKUOKA

We isolated the 5’ upstream promoter regions of the fruit-type sucrose synthase (SS) gene from tomato and Japanese pear by inverse PCR. The 5’ region of the tomato SS gene (TOMSSF) contained an intron approximately 1.6 kbp long in the 5’untranslated region, whereas the equivalent 5’ region in the Japanese pear SS gene (PypSUS1) had no intron. Each region was fused to the β-glucuronidase (GUS) g...

Journal: :iranian journal of biotechnology 2008
shahrokh gharanjik ahmad moieni amir mousavi houshang alizadeh

haploid microspore-derived embryos (mdes) of wheat were obtained by in vitro androgenesis. these  embryos were employed to evaluate the transient expression of gus gene (uida) following particle bombardment. using the bio-rad pds-1000/he system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0....

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